Genomic DNA was extracted from individual mosquitoes using cetyl trimethyl ammonium bromide (CTAB) 2% method described in Yahouedo et al. . Species identification of the An. gambiae complex members were done as per Scott et al.  and Favia et al. . The presence of L1014F and L1014S kdr mutations were assessed by PCR using the method described by Martinez-Torres et al.  and Ranson et al. . The PCR-RFLP diagnostic test was used to detect G119S ace-1R mutation as described by Weill et al. .
Brazilian Jiu Jitsu (BJJ) is a martial arts sport focusing on grappling and fighting. In the Japanese language, Ji means gentle while Jitsu means art. BJJ started as a gentle art used for self-defense but has since evolved into a deadly martial art.
Judo aims to submit an opponent with a choke, pin them to the ground or throw them. There are 3 basic categories in Judo, the striking (atemi-waza), grappling (katame-waza), and throwing(nage-waza) techniques. However, grappling and throwing are the two most used methods.
It teaches them grappling, joint locks, combat techniques, kicks, and more. The United States Marine Corps cultivated 17 deadly fighting styles from lethal martial arts like Krav Maga, Brazilian Jiu Jitsu, Muay Thai, and Taekwondo to develop MCMAP.
And has its history dating back to the early centuries in the Pyu Empire of Myanmar. Those days, the fighters wrapped their hands with hemp and continued fighting till one was knocked out or too injured to continue.
The Oyama karate school was considered one of the hardest Karate schools in Japan then. The training methods are brutal and more dangerous than any other karate style. As a result, a lot of students injured themselves.
Bacom has a lot of similarities with Krav Maga as both involve vicious techniques and the use of weapons. The martial art focused on several combat and fighting techniques, including joint lock, choke holds, kicks, striking sensitive areas, and other bone-crushing movements.
In the 1940s, Kajukenbo was fast recognized in Hawaii as a method of self-defense against street criminals. Instead of carrying weapons, the indigenes acquainted themselves with Kajukenbo styles to defend themselves and leave their attacker brutally injured.
Kajukenbo was a mixture of almost all fighting techniques, from grappling and kicking to grabbing and punching. Self-defense movements and kicks were chosen from the five martial arts to give a practitioner the ability to effectively protect themselves in street fighting.
Often referred to as the Keysi Method, KFM combines several fighting techniques to develop a brutal fighting style. It mostly involves grappling and striking with more emphasis on striking and using the elbow.
Kung Fu comprises many fighting styles categorized according to their similarities or origin. The Chinese developed these martial arts more than 4000 years ago as a self-defense technique. The martial arts were also important in their rigorous army training as they considered hand-to-hand combat a very effective fighting skill.
Despite being an effective and old sport, wrestling is inarguably one of the most lethal sports ever. It is one of the fighting techniques that the Gladiators used during the Roman fight, and we all know how violent it ended.
History had it that Rough and Tumble was introduced to North America from England, where the sport was referred to as bragging and fighting. The combat sport is so brutal that most players end up blinded or forever maimed after the fight.
In the late 1700s, attempts were made to soften the brutal fighting techniques of rough and tumble in Virginia. Legislations were made to prohibit attacking eyes, biting, battering, and clutching of genitals. Despite these laws, the Southern highlands continue to practice this deadly combat sport.
Though the North American rough and tumble still exists, South Africa has developed an independent fighting style. Rough and Tumble in South Africa started in the 1980s when officials needed to train their soldiers in unarmed yet violent combat.
Krav Maga is the deadliest martial art in the world. The military self-defense art was developed for the Israeli Defence Force and Israeli Security forces. The lethal martial art includes Judo, KarateKarate, wrestling, boxing, and other brutal fighting techniques. 1e1e36bf2d